期刊简介
主要报道和刊登国内外、特别是我国消化病学者具有创造性的、有较高学术水平的基础和临床研究论文、研究快报等. 对具有中国特色的研究论文, 如食管癌、胃癌、肝癌、大肠癌、病毒性肝炎、幽门螺杆菌、中医中药、中西医结合和基于作者自己研究工作为主的综述性论文, 将优先发表. 读者对象为基础研究或临床研究的消化专业工作者。
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首页>世界华人消化杂志
- 杂志名称：世界华人消化杂志
- 主管单位：华人消化杂志；新消化病学杂志
- 主办单位：山西省科学技术厅
- 国际刊号：14-1260/R
- 国内刊号：14-1260/R
- 出版周期：旬刊
期刊荣誉：国家期刊奖百种重点期刊（第二和第三届）期刊收录：上海图书馆馆藏, 国家图书馆馆藏, 文摘与引文数据库, 维普收录(中), 医学文摘, 知网收录(中), CA 化学文摘(美), 万方收录(中)
含CD基因的人大肠癌特异性逆转录病毒载体的构建
崔龙；曹广文；王元和；屠岳；孟荣贵；高军；仇小芳；吴宗娣；谢苏庆
关键词：colorectal carcinoma, cytosine deaminase gene, retroviral vector, carcinoembryonic antigen, transcriptional regulatory sequence
摘要：AIM In order to express suicide gene specifically in colorectal carcinoma cells, we constructed the recombinant retroviral vector promoted by transcriptional regulatory sequences of carcinoembryonic antigen (CEA) gene.METHODS A 1.3 kb EcoRⅠ/ BamHⅠ fragment of the CD gene was inserted into pUC118, and then the HindⅢ/ EcoRⅠ CD fragment was isolated. A 420 bp HindⅢ/ BamHⅠ fragment from pCEA 424/ 2CAT was released and subcloned into pUC118 to harvest HindⅢ/ EcoRⅠ CEA promoter. These two fragments were ligated with the linearized retroviral vector GINa using T4 DNA ligase.RESULTS Restriction enzyme SalⅠ was used to digest the recombinants to identify a positive clone A7 and a 1.7 kb band was observed on agarose electrophoresis gel. The recombinant retroviral vector, GICEACDNa was constructed.CONCLUSION Success in construction of GICEACDNa provides a basis for colorectal carcinoma-specific gene therapy.
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